•Using the nuclei
form cryopreserved germ cells or
gametes, derived directly from males, and fertilizing a enucleated egg to produce an androgenote. The resulting embyro is then shocked (heat or pressure) to result in a homozygous diploid due to suppression of first mitotic division.
•Major advantage
of this method is that all progeny
are genetically different and sperm are easy to collect and store.
•Disadvantages. Uncovers genetic recessive mutants